Why is sterilization repeated for each step of the culture method?
Bacteria . . .?
It is absolutely to avoid contamination. For example, if you are trying to amplify a plasmid that you constructed by PCR, then you do not want other unwanted bacteria (that you cannot determine which bacteria have the plasmid or not) on your plate or in your LB. You do not want your hard work going down the drain becuase of some contamination that could have been prevented.
good luck.
Reply:to avoid contamination.
Reply:To not let germs get in.
Reply:to ensure its effectiveness....
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