Best Bacteria/ Antibiotics to Use in Experiment??

Hi Everyone!!


I have to conduct an experiment involving antibiotic resistance...I'd like to do both of the following...


-Test one antibiotic on three or four bacteria types to see which are resistant, etc...


and


-Test two or three antibiotics on the same three or four bacteria (although not at the same time to maintain a control variable) to test for which antibiotic works best each strain, which is the most cosmoploitan, etc...





I was hopig to use agar plates, which I would seed with bacteria and then apply antibiotic.





Which bacteria and antibiotic types do you think would be best for use? I can't use any "dangerous" bacteria and I'd love it if the antibiotics are easy to maintain and (as a bonus) reputed to have resistance gained against it by many bacterial types.


Thanks!!!

Best Bacteria/ Antibiotics to Use in Experiment??
There are a great number pathogenic bacteria that affect animals other than man. Bacillus subtilis is just one of many. You can use non pathogenic bacteria too. (many of them have resistance genes too. Such resistance genes are thousands of years old. (e.g. a bacterium in a 3,000 year old mummy had resistance to tetracycline. I would use a number of antibiotics having different modes of activity. ( e.g. penicillin v or g for cell wall synthesis, aminoglycosides for protein and ribosome disruption, also clindamycin that attacks another step in the synthesis cycle, D.N.A. gyrase disruptors such as the fluoroquinone family (like ciprofloxacin,ofloxocin etc.). You could also use different varieties oif the same type like first generation penecillin (e.g. pen. g), second generation (e.g. ampicilin), third generation ones like monobactams, and carbapenems. ) and compare their activity. (also, penicillin with suicide inhibitors, (e.g. ampicilin like ampicilin plus clavulanic acid or, tazobactam or sulbactam.) Suggestion, contact a medical lab at a hospital, or university. I'm certain that they would most happy to help with the experimental design and bacterial cultures and antibiotcs.


Good luck.


Happy Holidays!


Doc. Dan.
Reply:You're most welcome.


Have a wonderful, happy holiday!


Dan. Report It

Reply:fluoroquinone should read fluoroquinolone. D.N.A. is also known as D.N.A. topoisomerase, For bacteria. Report It

Reply:fluoroquinone should read fluoroquinolone. D.N.A. is also known as D.N.A. topoisomerase, For bacteria. Report It